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Abstract
Objective: This study was performed to determine the effect of tocotrienol rich fraction (TRF) on the expression of synaptogenic genes and proteins in aging C.elegans . Method: Age synchronized DP132 strain worms were divided into two groups; control and treatment where worms were grown in nematode growth medium enriched with 50 ?g/ml TRF. The RNA and proteins expressions of syd-2, sad-1 and aex-3 were determined at day 2, 8 and 13 by q-RTPCR and western blot respectively. Results: The RNA and proteins expressions of syd-2, sad-1 and aex-3 decreased significantly with age in C.elegans . Interestingly, TRF treatment successfully increased the expression of these molecules from day 2 to day 8 which is known to be the reproductive phase of C.elegans but showed limited effect on the expression of these proteins at day 13. Conclusion: TRF enhances the expression of molecules involved in synaptic function of young C.elegans .
Highlights
Regional cognitive decline is one of the arising concerns in developing countries as the number of elderly people with biological brain aging is expected to rise[1]
Day ap
TRF treatment effectively increased the expression of SAD-1, SYD2 and AEX-3 proteins which are involved in synaptic information transmission
Summary
Regional cognitive decline is one of the arising concerns in developing countries as the number of elderly people with biological brain aging is expected to rise[1]. Alternative approaches have been emerging to improve cognition Antioxidants such as vitamin E have been postulated to be pivotal as it is able to protect the brain from oxidative stress[9][10][11]. Tocotrienols are gaining much attention due to their potent antioxidant and neuroprotective properties. Presence of this unsaturated side chain allows tocotrienols to penetrate into tissues efficiently enables tocotrienols to have far reaching and efficient free radical scavenging properties compared to tocopherols[12][13]. The importance of synapse plasticity in cognition and advancing effects of tocotrienol in neuroprotection. The worm has a complete sequenced genome which enables RNA interference and gene knockout advancement[16]
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