Abstract
Objective: The study was done to determine the effect of Tocotrienol rich fraction (TRF) on the expression of RNAs in C. elegans under oxidative stress.
 Methods: The nematodes were divided into 4 groups and treated accordingly: control; TRF; hydrogen peroxide (H202); TRF treatment before and after H202-induction (TRF+H202+TRF). Expressions of RNAs were analyzed with Affymetrix Genechip C. elegans Genome Array and Genespring GX11 software where differentially expressed genes were further analyzed using gene ontology (GO). Selected genes (unc-15, cit-1.2, ftn-1, rsks-1, unc-4 and daf-12) were analyzed with RT-qPCR to validate the results.
 Results: TRF modulated the expression of 314 genes involved in determination of adult lifespan, regulation of growth and lipid modification. A total of 440 genes involved in RNA metabolic processes, transcription, growth and differentiation of muscle and nerve cells were differently expressed following H202 induction. TRF treatment before and after H202- induction resulted in 438 differentially expressed genes involved in RNA metabolic processes, transcription, response to xenobiotic stimulus and protein amino acid phosphorylation.
 Conclusion: TRF modulates the expression of genes involved in the regulation of lifespan in C. elegans.
 Bangladesh Journal of Medical Science Vol.18(4) 2019 p.711-721
Highlights
One unique feature in organism life cycle is the aging process
Hydrogen peroxide (H2O2) is one of the most abundant ROS in living cells 2. It is produced as a by-product of aerobic metabolism and it has been demonstrated to be involved in apoptosis pathway, induction of intracellular oxidative stress and acceleration of aging 3,4
All samples were in good quality and the hybridization process was done properly as shown by the consistency of profile intensity plots generated and there was no deviation of signal from the expected intensity profile
Summary
Goon Jo Aan[1], Noralisa Abdul Karim[2], Mohd Shahril Aszrin Zainudin[3], Suzana Makpol[4]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
