Abstract
<i>Withania somnifera</i> (Ashwagandha) root extract is very popular ancient herbal medicine. The objective of the study was to characterize and evaluate the impact of The Trivedi Effect<sup>®</sup> - Energy of Consciousness Healing Treatment (Biofield Energy Healing) on phytoconstituents present in the ashwagandha root extract using GC-MS and NMR. Ashwagandha root extract was divided into two parts. One part was denoted as the control, while the other part was defined as The Trivedi Effect<sup>®</sup> - Biofield Energy Treated sample, which received The Trivedi Effect<sup>®</sup> - Energy of Consciousness Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The GC-MS data indicated that the peak height and peak area of The Trivedi Effect<sup>®</sup> treated sample were found to be altered compared with the control sample. The peak height of the phytoconstituents present in the treated ashwagandha sample was altered significantly in the range of -8.32% to 89.25% compared with the control sample. Similarly, the peak area of the treated sample was altered significantly in the range of -4.28% to 216.30% compared with the control sample. Overall, the change in the peak area% of the treated sample was significantly altered in the range of -18.29% to 170.18% compared with the control sample. The GC-MS and NMR analysis results identified the presence of withanolides such as glyco-withanolides, alkaloids, and sugars in the root extract in both the sample. The peak area of 2,3,4,5-tetrahydropyridazine (1), methyl ethyl sulfoxide (2), 5,6-dihydro-2-methyl-4(H)pyran-3,4-dione (4), diethoxy-2-methyl-propane (5), 2,3,4,5-tetrahydroxy-tetrahydro-pyran (6), and 3,4-dimethyl-2(3H)-furanone (7) were significantly increased by 170.18%, 58.21%, 7.74%, 139.50%, 23.16%, and 45.63%, respectively in the treated sample compared with the control sample. On the contrary, the peak area% of 2-hydroxy--butyrolactone (3) was decreased by -14.96% in the treated ashwagandha compared with the control sample. From the results, it can be hypothesized that The Trivedi Effect<sup>®</sup> - Biofield Energy Treatment might have the impact on the intrinsic physicochemical properties of the phytoconstituents present in the ashwagandha root extract and responsible for the alteration in the relative peak height/area of treated sample compared with the control sample. As a result, the concentrations of the phytoconstituents assumed to be increased in treated sample compared with the control sample. This treated ashwagandha root extract would be helpful for designing better nutraceutical/pharmaceutical formulations which might be providing a better therapeutic response against autoimmune diseases, nervous and sexual disorders, infectious diseases, antiaging, diabetes, cancer, immunological disorders, stress, arthritis, etc.
Highlights
Now-a-days herbal medicines have been getting exploring throughout the world for the prevention and treatment of various diseases because of their impressive therapeutic effects and fewer side effects compared with the allopathic medicines [1]
The total ion chromatograms (TIC) having the chromatographic peaks with the retention times are shown in Figure 1, which further helped for the qualitative comparison between the treated and untreated samples
The change in the peak area% of the treated sample was significantly altered in the range of -18.29% to 170.18% compared with the control sample
Summary
Now-a-days herbal medicines have been getting exploring throughout the world for the prevention and treatment of various diseases because of their impressive therapeutic effects and fewer side effects compared with the allopathic medicines [1]. W. somnifera is mostly used in the herbal drugs and nutraceuticals for the prevention and treatment of various diseases such as nervous and sexual disorders, immunological disorders, infectious diseases, diabetes, cancer, ulcer, stress, arthritis, etc. The major active phytoconstituents of W. somnifera root extract contains highly oxygenated withanolides, alkaloids, numerous sitoindosides, withanamides, starch, reducing sugars, peroxidases, glycosides, dilcitol, withanicil, benzyl alcohol, 2-phenyl ethanol, benzoic acid phenyl acetic acid, 3,4,5-trihydroxy cinnamic acid, etc. A new proprietary herbomineral formulation was formulated that consisted of the herbal ashwagandha root extract along with minerals like zinc, magnesium, and selenium. This herbomineral formulation was designed as a nutraceutical supplement and can be used for the prevention and treatment of various human disorders
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