Invitro Antibacterial, Antioxidant and XRF Analysis of <i>Commelina Diffusa Burm.F. </i>Plant Extracts

Abstract

Ethiopia has a long history of using medicinal herbs for treating both human and animal illnesses. Nonetheless, not enough research has been done on the antibacterial properties and possible bioactive components of the majority of medicinal plants. Therefore, this study deals with the evaluation of phytochemical, antimicrobial, antioxidant activities, phenol content and XRF analysis of <i>Commelina Diffusa Burm.F. </i>plant extracts. Mean values of the antimicrobial activity, MIC, antioxidant activities, phenol content and XRF analysis were reported as mean ± standard deviation. The chloroform leaf extracts of the plant gave the highest yield 23.4% followed by methanol 22.27%. The presence of several metabolite components, including alkaloids, diterpenes, flavonoids, glycosides, phenol, protein, saponin, steroids, tannins, terpenoids, tri-terpenoids and amino acids, has been shown by qualitative phytochemical analysis of plant parts. Significant antibacterial activity against the test bacterial strains was demonstrated by steam extracts of<i> Commelina Diffusa Burm.F. </i>Moreover, the methanolic extract of the plant demonstrated notable antioxidant activity. The highest value of phenolic content was obtained in <i>Commelina Diffusa Burm.F. </i>steam methanol extract followed by leaf extract while <i>Commelina Diffusa Burm.F. </i>root extract shows lower phenolic content. In this study, threaten elements were determined in the <i>Commelina Diffusa Burm.F. </i>plant part by using XRF spectroscopy. Overall, this research contributes to the understanding of pharmacological potential of <i>Commelina Diffusa Burm.F. </i>and highlights the importance of further exploring its medicinal properties. The findings provide valuable insights into utilizing medicinal plants for disease treatment and support the development of natural therapeutic agents.