Effect of the drug on the oxidation stress and antioxidation condition in type 2 diabetes mellitus

Abstract

Objective To investigate the effect and mechanism of pioglitazone on oxidation stress and antioxidation conditions in patients of type 2 diabetes mellitus (T2DM). Methods Seventy patients of T2DM, diagnosed according to the standards of WHO,1999 ,were divided randomly into treated group(n=35) and control group(n=35). Patients of the former group were treated with insulin and pioglitazone ,while the latter were given insulin only to control blood glucose level. In other normal therapies, ACEI, statins, Vitamin C and Vitamin E were excluded. All patients were treated for 3 months. Fasting blood samples were got before and after treatment and reserved for measurement of NT, 12 (S)-HETE, GSH and GSH-PX. Results Sixty-five patients underwent the whole observation. The level of NT and 12(S)-HETE decreased markedly after treatment in both groups(P<0.01) ,with more significant in treated group than in control group (P<0.01 and P <0.05). The levels of GSH and GSH-PX elevated markedly after treatment in beth groups( P<0.01 and P<0.05 ,respectively) ,with more significant in treated group than in control group (P<0.01 and P<0.05 ). No adverse reactions were found during the treating process. Conclusion Pioglitazone shows antioxidant potency safely independent of glucose-lowering effect by decreasing of level of oxidation stress NT and 12(S)-HETE and increasing of level of antioxidant GSH and GSH-PX. Key words: Type 2 diabetes mellitus; Oxidation stress; Pioglitazone; Nitrotyrosine; 12 (S) - Hydroxyeicosatetraenoic acid; Glutathione ; Glutathione peroxiduse