Effect of superfine pulverization technology on the determination of vitamin C content in Fructus rosae laxae

Abstract

Superfine pulverization and ordinary pulverization were used to obtain powders with different particle sizes in different parts of Fructus rosae laxae. High-performance thin-layer chromatography (HPTLC) was used to carry out a simple, fast, qualitative, and quantitative analysis of vitamin C in Fructus rosae laxae. The effects of superfine pulverization and ordinary pulverization on the vitamin C content in the whole fruit, pulp, and seed of Fructus rosae laxae were compared. A G F254 silica gel thin-layer plate was utilized, and ethyl acetate, anhydrous ethanol, and water in (10:6:3.75, v/v) ratio were used as a developer. The scanning wavelength was 273 nm. Results showed that the Rf value of vitamin C was 0.61, the linear range was 0.25–1.2 μg, and the correlation coefficient was 0.9959, indicating good linear correlation. Precision RSD was < 2%, indicating good instrument precision. The sample was stable within 40 min at room temperature, and the RSD was < 3%. The average recovery of vitamin C was 98.39%, and the established method was reliable. The content of vitamin C in the whole fruit, pulp, and seed of Fructus rosae laxae after superfine pulverization was higher than that obtained from ordinary pulverization. The content of vitamin C in the pulp was the highest after superfine pulverization.