Abstract
Background: Fenugreek is used in the treatment of many diseases as reported in Ayurveda. The chemical like Diosgenin is one of the important chemicals of fenugreek. Aim: To develop simple, rapid, accurate methods for estimation of Diosgenin from fenugreek using high performance thin layer chromatography (HPTLC) and high performance liquid chromatography (HPLC). Materials and Methods: The two chromatographic methods, HPTLC and HPLC, were developed and compared to quantitative determination of Diosgenin in fenugreek seed extract. For both the analyses, the scanning wavelength was set at 205 nm. The Diosgenin content of seven different extracts collected from different locations was compared by HPLC and HPTLC. Statistical Analysis: The relative standard deviation and correlation coefficient (r2) were calculated by standard Formulae. Results: The comparison was done in HPTLC and HPLC results and all the results are found to be within the limits and showed no significant difference. Conclusion: Both the methods were accurate and specific for the analysis of Diosgenin. Key words: Diosgenin, fenugreek, high performance liquid chromatography, high performance thin layer chromatography, quantitation
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