Phytochemical Investigation and Anti-angiogenic Examination of Iraqi Vigna radiata L. Seeds and Sprouts

Rabab H Qassim,Enas J Kadhem

doi:10.31351/vol29iss2pp37-47

Abstract

Abstract  The objective of this study was to investigate the phytochemical constituents of two different parts of Vigna radiata (seeds and sprouts), and identify their anti angiogenic activity .the goal was achieved by Preliminary qualitative phytochemical screening for crude ethanolic extract of two parts of plant ; rat aorta anti-angiogenesis assay had been conducted for both extracts . isolation , separation and purification of some phytochemical constituents that belong to important groups (flavonoids) from n-butanol fraction extract of Vigna radiata plant had been done in pure form by using preparative thin layer chromatography ( PTLC ) and then subjected to different spectral analytical and physicochemical techniques to identify their chemical structure : melting point ( M. P) , thin layer chromatography ( TLC ) high performance liquid chromatography ( HPLC ) , Fourier transforms infrared spectra ( FTIR ) . and high performance thin layer chromatography ( HPTLC ).the result showed that the existence of coumarin , alkaloids, phenols, flavonoids , terpenoids, phytosterol and saponin.Vitexin and isovitexin were isolated in pure form .n-butanol fraction of sprouts showed highest percentage of blood vessels inhibition ( 88%) in comparison to n-butanol fraction of seeds which showed (56%). There IC50 were 58.8µg/ml and 56.6 µg/ml respectively. The difference of anti angiogenic activity may be related to the variation of concentration of the bioactive constituents or appearance of new bioactive constituents during germination.  Keywords: Vigna radiata, vitexin, isovitexin ,high performance thin layer chromatography HPTLC , high performance liquid chromatography HPLC

Highlights

Natural compounds provide a large of phytochemical constituents, thought to be the main antioxidants which act as radical scavengers and help in converting the radicals to less reactive species
The results showed that the most two important flavonoids that present in this plant are the vitexin and isovitexin
The results indicated that HPTLC method was developed for the first time for qualitative identification from seeds of mung bean plant in which qualitative identification was made by the comparison of maximum retardation factor ( Rf ) and UV spectrum of ethyl acetate fraction and n-butanol fraction of seeds and sprouts with standards of vitexin and isovitexin and isolated compounds R1,R2 as shown in Figures (7 -10)

Summary

Introduction

Natural compounds provide a large of phytochemical constituents, thought to be the main antioxidants which act as radical scavengers and help in converting the radicals to less reactive species. Vigna radiata L. plant known mung contributor factors to the antimicrobial, antioxidant, antitumor activity [4,5]. Flavonoids are the important metabolites found in mung bean [6,7]. Belong to Fabaceae family and mung bean seeds improves the antioxidant activity is native in China, India Bangladesh, South East and Western countries [1,2]. Mung beans contain balanced nutrients, including protein and dietary and elucidate important metabolites level for better usage. Germinating (sprouting) of mung bean by breeding or usage some hormones [8]

Objectives

Methods

Results

Conclusion