Abstract
The objective of this work was to determine the sodium metabisulphite (NaMBS) subchronic toxicity used as a food additive in Algeria. Three groups of female Wistar rats were treated with 0.25%, 1% and 4% of NaMBS in their drinking water for 90 days. An immunization protocol was conducted during the experiment. Mortality, comportmental and weight modifications, and food and water consumption were recorded. At the end of the experiment, the control and experiment rats were killed, and their blood and organs were removed. Immunoglobulin levels were evaluated; biochemical and hematological parameters were investigated. Our results showed that the administration of NaMBS at 1% and 4% had significant effects on body weight, food and water consumed. There was an increase in biochemical parameters (calcium, urea, creatinine, uric acid, transaminases) and decrease immunoglobulin levels. The hematology revealed a decrease in red blood cells and hemoglobin, as well as leucocytosis. Physiological study showed enlarged spleen, kidney, liver and stomach. In light of our results, we can conclude that subchronic intake of NaMBS 1% and 4% seems to alter immune function, biochemical, hematological and physiological para-meters in Wistar rats.
Highlights
Sulphites are substances naturally present in foods and in the body
The animals were separated into four groups (n = 6) and received daily three different doses of NaMBS (0.25%, 1%, and 4%) in water for 12 weeks, the doses chosen are based on the value of acceptable daily intake ADI [12]
The hematologic analyses were determined on the same day after the blood samples were taken from the rats,using automated Advia 60 CT (Bayer DC): white blood cells, red blood cells, hematocrit; haemoglobin was determined by the colorimetric method of Drabkin [14]
Summary
Sulphites are substances naturally present in foods and in the body. They are regulated food additives thatHow to cite this paper: El Kadi, F.Z., Bénali, A.I., Bénali, M. and Belbraouet, S. (2014) Effect of Sodium Metabisulphite on Blood Metabolic Status of Wistar Rats. Sulphites are substances naturally present in foods and in the body. They protect the preparations against enzymatic or non-enzymatic browning [1]. Endogenous sulphites are normally generated in the human body by degradation of amino acids such as methionine and cysteine-sulphur. They can be generated by polymorphonuclear neutrophils [3]. Exogenous sources of sulphites include food, drinks, ambient air and pharmaceutical products [5]
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