EFFECT OF SEA-BUCKTHORN ALCOHOLIC EXTRACTS (HIPPOPHE FRUCTUS ) ON HEPATIC AND RENAL FUNCTIONS IN LABORATORY RAT

Abstract

In order to observe the blood biochemical modifications as an indicator of hepatic and renal dysfunctions that results after treatment with supplementary copper doses in feed, 48 weaned, male Wistar rats were studied, during a period of three weeks. Primary objective of the research was to observe the blood biochemical modifications in case of copper associated pathology on the base of the activity of some enzymatic systems, modified as a consequence of overdosing copper products in rats, and the influence of alcoholic extracts from sea-buckthorn fruits (Hppophae Fructus) on these parameters, knowing its important antioxidant activity, due to the high content of ascorbic acid, tocopherols, polyphenols, carotenoids and selenium. In rats, hepatic and kidney toxicity was evidently indifferent of the copper dose added in feed, and it was expressed by the increased activity of serum transaminases and alkaline phosphatase, associated with an increased level of blood nitrogen urea and creatinine levels. The studied enzymes activities indicated changes in the hepatic and renal functions, confirming the toxic action of copper, registering lower levels of investigated enzymes activity in the case of alcoholic extracts from sea-buckthorn fruits (Hppophae Fructus) supplementary administered into feed that show a significant antioxidant action, which reduce the risks.