Abstract
Anorganic bovine bone matrix (Bio-Oss®) has been used for a long time for bone graft regeneration, but has poor osteoinductive capability. The use of recombinant human bone morphogenetic protein-2 (rhBMP-2) has been suggested to overcome this limitation of Bio-Oss®. In the present study, heparin-mediated rhBMP-2 was combined with Bio-Oss® in animal experiments to investigate bone formation performance; heparin was used to control rhBMP-2 release. Two calvarial defects (8 mm diameter) were formed in a white rabbit model and then implanted or not (controls) with Bio-Oss® or BMP-2/Bio-Oss®. The Bio-Oss® and BMP-2/Bio-Oss® groups had significantly greater new bone areas (expressed as percentages of augmented areas) than the non-implanted controls at four and eight weeks after surgery, and the BMP-2/Bio-Oss® group (16.50 ± 2.87 (n = 6)) had significantly greater new bone areas than the Bio-Oss® group (9.43 ± 3.73 (n = 6)) at four weeks. These findings suggest that rhBMP-2 treated heparinized Bio-Oss® markedly enhances bone regeneration.
Highlights
For dental implant treatment, primary surgical augmentations are often required due to the lack of alveolar bone at surgical sites [1]
The surfaces morphologies of Bio-Oss®, heparinized Bio-Oss® and heparinized recombinant human bone morphogenetic protein-2 (rhBMP-2)-Bio-Oss®
We confirmed that green fluorescent protein (GFP)-conjugated rhBMP-2 was immobilized on heparinized Bio-Oss® surfaces
Summary
Primary surgical augmentations are often required due to the lack of alveolar bone at surgical sites [1]. The purpose of this procedure is to obtain sufficient vertical and horizontal volumes of alveolar bone. Alveolar bone augmentation by autogenous block bone grafting is recognized as a gold standard [2]. Amounts of block autogenous bone graft are limited at donor site, and an additional bone graft donor procedure is required, which increases the impact of surgery and introduces the risk of postoperative complications, such as, infection, bleeding, pain, edema, and damage to nerves and blood vessels [3]. Bio-Oss® (Geistlich, Wolhusen, Switzerland) is a deproteinized bovine bone comprised of calcium-deprived carbonate apatite with the same mineral content as human bone [5]
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