Abstract
BackgroundThe aim of this study was to determine the osteoconductivity of hydroxyapatite particles (HAP) as a carrier for Escherichia coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2). Two 8-mm diameter bicortical calvarial defects were created in each of 20 rabbits. One of each pair of defects was randomly assigned to be filled with HAP only (HAP group) or ErhBMP-2 loaded HAP (ErhBMP-2/HAP group), while the other defect was left untreated (control group). The animals were killed after either 2 weeks (n = 10) or 8 weeks (n = 10) of healing, and histological, histomorphometric, and tomographic analyses were performed.ResultsAll experimental sites showed uneventful healing during the postoperative healing period. In both histomorphometric and tomographic analyses, the new bone area or volume of the ErhBMP-2/HAP group was significantly greater than that of the HAP and control groups at 2 weeks (p < 0.05). However, at 8 weeks, no significant difference in new bone area or volume was observed between the ErhBMP-2/HAP and HAP groups. The total augmented area or volume was not significantly different between the ErhBMP-2/HAP and HAP groups at 2 and 8 weeks.ConclusionsCombining ErhBMP-2 with HAP could significantly promote rapid initial new bone formation. Moreover, HAP graft could increase new bone formation and space maintenance, therefore it might be one of the effective carriers of ErhBMP-2.
Highlights
The aim of this study was to determine the osteoconductivity of hydroxyapatite particles (HAP) as a carrier for Escherichia coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2)
Our research group recently succeeded in producing BMP-2 using an Escherichia coli production system (ErhBMP-2), which is attractive for biotechnology because of the ability of E. coli to grow rapidly and to high density on inexpensive substrates [6]
We aimed to evaluate the effect of HAP on bone regeneration and to determine the efficacy of HAP as a carrier for ErhBMP-2 in the rabbit calvarial intraosseous defect model
Summary
The aim of this study was to determine the osteoconductivity of hydroxyapatite particles (HAP) as a carrier for Escherichia coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2). BMP-2 derived from Chinese hamster ovary cells (CHO BMP-2) is relatively costly because protein yields are low In this context, our research group recently succeeded in producing BMP-2 using an Escherichia coli production system (ErhBMP-2), which is attractive for biotechnology because of the ability of E. coli to grow rapidly and to high density on inexpensive substrates [6]. HA is used as a bone graft extender for posterolateral spinal fusion in humans [12] It is useful as an ErhBMP-2 carrier because of its high affinity for ErhBMP-2. In another study, alkaline phosphatase activity was significantly higher in mandibular defects treated with porous HA and ErhBMP-2 than in controls treated with HA alone at both 7 and 21 days [13], indicating that ErhBMP2 accelerated bone formation by osteoconduction from porous HA
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