Effect of resveratrol on behavioral, biochemical, and immunological parameters of DBA/2J and tetrahybrid DBCB mice receiving diet with excess fat and fructose

Abstract

Polyphenolic biologically active substances (BAS) including resveratrol (R) can exert beneficial effects on fat accumulation, blood pressure, glycemia, insulin sensitivity, and plasma lipid profile in patients with obesity, and associated diseases. The study aimed to determine the effect of R at a dose of 25 mg/kg body weight on the DBA/2J and DBCB mice with diet-induced obesity followed by the consumption high-fat high-carbohydrate diet (HFCD). Behavioral reactions (elevated plus maze [EPM]) and muscle tone (the strength of the forepaw grip) were tested, and plasma biochemical and immunological parameters were assessed. In the repeated EPM test, anxiety increased only in DBCB mice during the second trial. In DBCB mice treated with HFCD, the muscle tone decreased with the second trial; however, this effect was not observed in the background of R consumption. R decreased the level of triglycerides, diminished the activities of alanine and asparagine aminotransferases, which were elevated upon HFCD consumption. Ghrelin level increased after R consumption in mice of both genotypes. The leptin to ghrelin ratio was reduced in DBCB mice receiving R. Consumption of R increased IL-3 and IL-10 levels in both DBA/2J and DBCB mice. IL-12p70 level increased in DBCB mice in response to R. R addition to HFCD reduced several symptoms of dyslipidemia in highly sensitive tetrahybrid mice. The results obtained indicate the importance of a personalized (depending on the genotype) approach when any R prescription, among other BAS and dietary factors, are used in diet therapy for patients with low, moderate and high-risk obesity.