Abstract
1. 1. The synthesis of hemoglobin was studied in a system in vitro which contained ribosomes, pH-5 enzymes, and the supernatant solution prepared from rabbit reticulocytes. 2. 2. When protoporhyrin IX was added to this “cell-free” system, the release into solution of protein labeled with [ 14C]leucine was increased 2- to 3-fold. 3. 3. The same effect was observed whether the protoporhyrin IX was added initially or after the reaction mixture had been incubated for 10 min. 4. 4. The increase in the radioactivity of the soluble protein was maximal when the concentration of protoporphyrin IX was 32 μM. 5. 5. These studies suggest that the mechanism for the release of hemoglobin from the ribosomes may be associated with the synthesis of heme from protoporphyrin IX and iron.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
