Abstract
<p class="abstrak3"><span lang="IN">The study </span><span>was </span><span lang="IN">aimed to examine effect of protecting soybean meal using mahogany leaf extract on rumen fermentation products in vitro. The material used was cow rumen fluid, basal ration consisting of concentrate and elephant grass with a ratio of 60:40%, and mahogany leaves. The research was carried out in three stages: mahogany extraction, protein protection using mahogany extract, and in vitro stages. The test was conducted in vitro </span><span>based on</span><span lang="IN"> a completely randomized design (CRD). The treatments consisted of 4 kinds of soybean meal protection with 0% tannin concentration (</span><span>T</span><span lang="IN">0); 1.5% (</span><span>T</span><span lang="IN">1); 3% (</span><span>T</span><span lang="IN">2); and 4.5% (</span><span>T</span><span lang="IN">3). </span><span>D</span><span lang="IN">ata obtained were analyzed by analysis of variance and tested using orthogonal polynomials. </span><span>R</span><span lang="IN">esults showed that addition of protected soybean meal with mahogany leaf extract had a cubic</span><span>al</span><span lang="IN"> effect on partial VFA, methane gas and post-rumen dissolved protein, a quadratic effect on protozoa, and a linear effect</span><span>on </span><span lang="IN">N-NH<sub>3</sub>, SPM, and RUDP. Giving extra mahogany leaves at a level of 1.5% produced a fermented product that was not different from the control while giving a level of 3% got the highest fermentation product. Giving mahogany leaf extract at a level of 4.5% resulted in the highest SPM, and RUDP but there was a decrease in soluble protein, which indicated the occurrence of overprotection. The addition of 3% mahogany leaf extract effectively increased rumen fermentation products, RUDP, and soluble protein without disturbing the activity of rumen bacteria.</span></p>
Highlights
Soybean meal is a source of high-quality feed protein with a crude protein content of 49%
Waldi et al (2017) showed that soybean meal has a high in vitro solubility (60-80%) so that it is quickly degraded in the rumen and within 48 hours it’s protein degradation reached 24,149 gN/hour and was indicated by high levels of ammonia (12.97-14.42 mM)
Soybean meal protein that is resistant to degradation in the rumen ranges from 22-53%, while the digestibility of the rumen-resistent protein in the small intestine reaches 86-100% (Arisya et al 2019)
Summary
Soybean meal is a source of high-quality feed protein with a crude protein content of 49%. Waldi et al (2017) showed that soybean meal has a high in vitro solubility (60-80%) so that it is quickly degraded in the rumen and within 48 hours it’s protein degradation reached 24,149 gN/hour and was indicated by high levels of ammonia (12.97-14.42 mM). Waldi et al (2017) showed that soybean meal has a high in vitro solubility (60-80%) so that it is quickly degraded in the rumen and within 48 hours it’s protein degradation reached 24,149 gN/hour and was indicated by high levels of ammonia (12.97-14.42 mM). Soybean meal protein that is resistant to degradation in the rumen ranges from 22-53%, while the digestibility of the rumen-resistent protein in the small intestine reaches 86-100% (Arisya et al 2019). Soybean meal protection using formaldehyde has a weakness It can inhibit microbial activity in the rumen because formaldehyde dissolves and enters the rumen fluid (Suhartanto et al 2014), while protein protection by heating makes protein difficult to be digested (Mayangsari et al, 2013). Without protection was 96.4%, and the lowest protein digestibility was protection with formaldehyde at 66.3% (Stern, et al, 2006)
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