Effect of pinealectomy on the morphology of the chick cervical spinal cord: A stereological and histopathological study

Abstract

Melatonin has some effects upon morphological features of various structures in small animals and human being. In this study, the changes induced by pinealectomy procedure on morphological features of developing cervical spinal cord and their neurons in the gray matter (GM) and white matter (WM) of cervical spinal cord in the chicken were investigated. A total of 15 Hybro Broiler newly hatched chicks were randomly divided into three equal groups: unoperated control group ( n = 5), sham-operated group ( n = 5) and pinealectomy group ( n = 5). Pinealectomy procedure and sham operation were done in 3-day-old chicks and all animals were sacrificed at the 8th week and the 6th cervical (C6) spinal cord segment was dissected out for histopathological evaluation and subsequent stereological analysis. The volume of spinal cord segment did not show a significant difference between unoperated and sham-operated controls, but the pinealectomy group has a declined volume value compared with those of the control and sham operated groups ( P < 0.01). By contrast, there was no statistically significant difference between unoperated and sham-operated controls and the pinealectomy group with regard to volume fraction of the GM and WM of the cervical spinal cord. Finally, it was observed that pinealectomy procedure significantly reduces neuron number in the GM and the volume of WM of the C6 segment of cervical spinal cord in the chicken ( P < 0.001). The present study is the first study at all to evaluate the effects of pinealectomy on quantitative feature of the spinal cord in the chicken. Based on our findings, we suggest that pineal gland/melatonin might play an important role in morphological features of the developing spinal cord in the chicken.