Abstract
In this study we investigated the effect of near-infrared fluorescent quantum dots (NIRF-QDs, QTracker) on the proliferation, adherence, invasion and chemotaxis of human tongue squamous cell carcinoma cell line Tca8113 in vitro. Cell proliferation and colony formation rate were determined by using a hemocytometer and culture plate. A transwell chamber assay was used to determine the cell invasion, adherence and chemotaxis. The results showed that there was no significant difference between the results of Tca8113 cells labeled with NIRF-QD800 and those of unlabeled Tca8113 cells, suggesting that the proliferation, invasion, adherence and chemotaxis of Tca8113 cells were not affected by NIRF-QD800. These results provide a basis for the further utilization of NIRF-QDs in non-invasive imaging and tracking of tumor cells in vivo.
Highlights
Dynamic and in situ monitoring the genesis, development, infiltration, metastasis and distribution of cancer cells in vivo are the keys for the investigation of tumor occurrence, advances, early diagnosis and treatment
We observed the proliferation, adherence and chemotaxis of the QD800-labeled Tca8003 cells. These results provided theoretical bases to reveal if the visualized tumor cells labeled by peptide-conjugated near-infrared fluorescent quantum dots (QDs) reflected the actual genesis, development, infiltration and metastasis of tumors in vivo
The results indicated that there was no significant difference between the growth curved of QDs-labeled and unlabeled Tca8113 cells, indicating that the presence of QD800 does not affect the growth of Tca8113 cells
Summary
Dynamic and in situ monitoring the genesis, development, infiltration, metastasis and distribution of cancer cells in vivo are the keys for the investigation of tumor occurrence, advances, early diagnosis and treatment. The primary technique to solve these problems is to develop an optical marker that is detectable and suitable for in vivo applications [1,2,3]. QDs have a high photoluminescence quantum yield, high intense fluorescence, excellent photostability and high resistance to photobleaching and photodegradation [4,5]. Their absorption spectra are wide, continuous and can be excited at any wavelength shorter than their emission peak. Their emission spectra are narrow, symmetrical and the effective Stokes shifts are large
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