Abstract
Abstract Objectives:To explore near-infrared fluorescent imaging in vivo using quantum dots Qdot800 and Alexa680 dye labeling Nimotuzumab for human tongue squamous cell carcinoma diagnosis, location. Methods: Near-infrared fluorescent quantum dots Qdot800 and Alexa680 dye were respectively conjugated with Nimotuzumab. Subsequently, the probes were used to detect fixed or live human tongue squamous cell carcinoma cell line Tca8113. After Tca8113 subcutaneous xenografts grown in, the nude mice were injected intravenously with one of the conjugates followed by fluorescent imaging using small animal imaging system. The images of targeting tumor in vivo were required and analyzed. Results: Near-infrared fluorescent quantum dots Qdot800 and Alexa680 dye were successfully conjugated with Nimotuzumab. The conjugates were able to specifically and efficiently recognize fixed and live Tca8113 with intense fluorescence. Although Nimotuzumab labeled with Qdot800 could not be detected targeting the subcutaneous Tca8113 xenografts grown in vivo, Nimotuzumab labeled with Alexa680 performed very well with bright fluorescence in contrast to surrounding normal tissue. Conclusions: The fluorescent conjugates probes Nimotuzumab conjugated with Near-infrared fluorescent Qdot800 and Alexa680 dye were able to specifically target human tongue squamous cell carcinoma cell in vitro. Alexa680 labeling with Nimotuzumab was more suitable than Qdot800 to perform fluorescent imaging in vivo for the study of human tongue squamous cell carcinoma. [Key words] tongue squamous cell carcinoma, fluorescent imaging, fluorescent probe, Nimotuzumab Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 2437. doi:1538-7445.AM2012-2437
Published Version
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