Abstract
One-year-old Scots pine ( Pinus sylvestris L.) seedlings were exposed to 0.1 and 0.15 ppm O 3 for four weeks, and to 0.3 ppm O 3 for six days in three separate experiments. Plants did not exhibit any visible injuries or differences in growth parameters. Ozone did not affect the numbers of nymphs produced by grey pine aphid [ Schizolachnus pineti (F.)]. The levels of total essential amino acids for aphids were not significantly affected by O 3. The levels of total amino acids were significantly reduced on exposure to 0.3 ppm O 3. Reduced levels of starch in shoots and roots were found in O 3-exposed seedlings. No changes in other sugars (glucose, fructose, sucrose) were detected. Ozone had no effect on secondary compounds: monoterpenes, resin acids, total phenolics and catechins. The results indicate that rising levels of atmospheric O 3 may disturb primary carbohydrate and amino acid metabolism of Scots pine, while secondary metabolites remain unaffected.
Published Version
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