Effect of microRNA-10a targeting MTMR3 on growth and migration of glioma cells

Abstract

Objective To investigate the effect of microRNA (miRNA, miR)-10a on the growth and migration of glioma cells and its molecular mechanism. Methods The expression levels of miR-10a in glioma and adjacent tissues were analyzed by fluorescence quantitative polymerase chain reaction (PCR). miR-10a overexpression cell line (experimental group) and control cell line (control group) were constructed in U251 glioma cell line. The proliferation and migration ability of glioma cells in control group and experimental group were determined by CCK-8 kit and Transwell. The target genes of miR-10a were analyzed by Bioinformatics and double luciferase reporter genes. The proliferation and migration ability of cells in experimental group cells that overexpressed target genes were analyzed. The difference between two groups were analyzed by t test. Results Compared with glioma adjacent tissues (1.01±0.19), the expression of miR-10a in glioma tissues (2.38±0.28) (t=3.918, P<0.05). The cell proliferation ability in the experimental group was significantly higher than that of the control group (F=2.991, P<0.05). Compared with the control group (48.67±13.42), the migration ability of tumor cells in the experimental group (128.32±19.32) increased significantly (t=4.109, P<0.05). Bioinformatics analysis showed that MTMR3 was the target gene of miR-10a. Compared with control cells (1.19±0.21), the expression level of MTMR3 protein in experimental cells (0.21±0.09) significantly decreased (t=3.819, P<0.05). Overexpression of MTMR3 protein significantly inhibited the proliferation and migration of glioma cells in miR-10a overexpression cells compared with those in overexpression control vectors (P<0.05). Conclusion MiR-10a is highly expressed in glioma and regulates the proliferation and migration of glioma cells by targeting MTMR3 protein. Key words: MicroRNA-10a; MTMR3; Glioma; Proliferation; Migration