Effect of Medium Composition on the Synthesis of Chitinase and Chitin Deacetylase from Thermophilic Paenibacillus sp.Hul

Abstract

To investigate the effects of nutritional components and initial pH on chitinase and chitin deacetylase production by thermophilic Paenibacillus sp. Hul, and provide basis for fermentation of enzymes and preparation of chito- oligosaccharides. The shake flask culture was employed to research the culture conditions of thermophilic Paenibacillus sp. Hu1 for producing chitinase and chitin deacetylase. The results show that the optimal carbon source was chitin powder with 35g/L; while yeast extract power was the optimal nitrogen source with 10g/L. It was beneficial to synthesize chitinase and chitin deacetylase when the initial pH was 6.0. In addition, the synthesis of chitinase and chitin deacetylase was inhibited by Cu2+, and activated by Ca2+. However, Mg2+ and Zn2+ did not show significant inhibitory or activation effects on the synthesis of the two enzymes. The hydrolysate components were analyzed using chitin as substrate with the crude enzyme, and the result suggested that enzymatic hydrolysates were mainly centralized in chito-oligosaccharides with polymerization number less than hexaose.