Solid state fermentation production of chitin deacetylase by Colletotrichum lindemuthianum ATCC 56676 using different substrates.

Abstract

Production of extracellular chitin deacetylase by Colletotrichum lindemuthianum ATCC 56676 under solid substrate fermentation was studied. The suitability of shrimp shell chitin waste (SSCW) and commercial wheat bran (CWB) was evaluated for maximal enzyme production. CWB medium (pH6.4 ± 0.2) supplemented with chitosan favoured maximal chitin deacetylase yield of 460.4 ± 14.7 unit/g initial dry substrate (U/g IDS) at 96h as compared to maximal yield of 392.0 ± 6.4U/g IDS at 192h in SSCW medium (pH8.7 ± 0.2) at 25°C incubation temperature and 60% (w/w) initial moisture content of medium. Along with chitin deacetylase, C. lindemuthianum ATCC 56676 produced maximum endo-chitinase (0.28 ± 0.03U/g IDS at 144h) and β-N-acetylhexosaminidase (0.79 ± 0.009U/g IDS at 192h) in CWB medium and 0.49 ± 0.05U/g IDS of endo-chitinase at 264h and 0.38 ± 0.04U/g IDS of β-N-acetylhexosaminidase at 96h of incubation in SSCW medium. SEM studies indicated the difference in the morphology of mycelia and hyphae of C. lindemuthianum ATCC 56676 when grown on different solid substrates. Production of chitin deacetylase by SSF is being reported for the first time.