Abstract
Optimization of the fermentation conditions for chitin deacetylase (CDA) production by Penicillium oxalicum SAEM-51 was undertaken in the present study using central composite design (CCD) under submerged condition. CDA is widely employed for bio-catalytic conversion of chitin to chitosan. Chitosan is a biopolymer with immense commercial potential in diverse industrial sectors, viz. pharmaceutics, food, agriculture, water treatment, etc. CDA production was significantly affected by all the variables studied, viz. pH, temperature, inoculum age and size. The optimal conditions that stimulating maximal CDA production were found to be: pH, 7.9; temperature, 28 °C; inoculum age, 90 h, and 11 % inoculum size. Under these optimized conditions, the actual maximal CDA production was 623.57 ± 8.2 Ul−1, which was in good agreement with the values predicted by the quadratic model (648.24 Ul−1), confirming the validity of the model. Optimization of fermentation conditions through CCD had resulted into 1.4-fold enhancement in CDA productivity (Qp = 4.3264 Ul−1 h−1). Results of these experiments indicated that response surface methodology was proved to be a promising method for optimization of CDA production.
Highlights
Modern era is the era of biotechnology for the production and application of various bio-based products
Optimization of the fermentation conditions for chitin deacetylase (CDA) production by Penicillium oxalicum SAEM-51 was undertaken in the present study using central composite design (CCD) under submerged condition
The effect of four culture variables on CDA production was determined by CCD
Summary
Modern era is the era of biotechnology for the production and application of various bio-based products. Recently commercial potential of chitin and its soluble derivatives has been explored a lot (Synowiecki and Al-Khateeb 2003; Dutta et al 2004; Aranaz et al 2009; Khoushab and Yamabhai 2010). The N-deacetylated derivative of chitin has enormous commercial potential due to its competitive properties like biodegradability, biocompatibility, solubility, non-toxicity, etc. Chitosan is present in small amounts as animal biomass in the shells or cuticles of many crustaceans and in the fungal cell wall, mostly it is derived from chitin by chemical or bio-catalytic alkaline deacetylation process. Chemical process needs a large amount of concentrated alkali, which in turn causes environmental concern. Alkaline deacetylation process leads to the degradation of product quality with generation of heterogeneous products (Chang et al 1997). Bioconversion is a competent alternative to the chemical one as it
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