Abstract
This experiment was conducted at the plant tissue culture laboratory – Dept. of Horti.- Coll. of Agricultural Engineering Sciences – Univ. of Baghdad, in order to study the effect of magnetic field in the propagation and production of volatile oil compounds of rosemary plant Rosmarinus officinlis. Factorial experiments within completely randomized design with thirty replications were used. The results indicated an increase in the treatment of exposure to south pole for 30 days with the highest mean of the shoots number, fresh and dry weight and total chlorophyll content (7.5 shoots.plant-1, 6634 mg, 769.5 mg and 160.6 mg.100 g-1) respectively. While the control treatment produced the lowest value of shoot number, fresh and dry weight and total chlorophyll content (5.7 shoots.plant-1, As shoots length, the exposure to south pole for 30 days, gave the shoot length (6 cm), while the exposure treatment to north pole for 20 days gave mean branch length of 2.25 cm. In terms of the effect of magnetic field on the concentration of volatile oil compounds, the results indicate that the exposure to south pole for 30 days significantly higher than the other treatments in the following compounds: linalool, Terpinene, linderol, Limonene cymene, and camphor penene. The 30-day north pole exposure was significantly higher in the two compounds: merycenen and sabinene.
Highlights
Rosmary (Rosmarinus officinalis L.) is a medicinal plant that is an perennial herb with fragrant that follows the Lamiaceae family, has dense growth and strong perpendicular spherical branches
The growth and development of the plant is influenced by the magnetic field as the plants respond to this field and the importance of the results obtained on the role of the magnetic field in improving the growth of field plants encouraged the study of the effects of magnetic properties in the growth of plant parts in vitro [20], exposed the parts of potatoes grown in vitro to a magnetic field with a magnitude of 6,4,2 militeslas, the intensity of 4 militeslas was the best in improving the growth rate and there is no effect of polarity
The percent of branches formations was 86.96% compared to 61.9% for non-magnetic exposure parts, and the duration of 2.2 seconds was the best in increasing fresh weight and concentration of chlorophyll exposed to the magnetic field compared to non- exposed
Summary
Rosmary (Rosmarinus officinalis L.) is a medicinal plant that is an perennial herb with fragrant that follows the Lamiaceae family, has dense growth and strong perpendicular spherical branches. Al-Bashi [2] studied the effect of the exposure period of the gardenia branches grown on MS medium to the magnetic field of the south or North Pole with intensity of 200 militeslas in the growth and multiplication of branches. Apical buds were taken from two-year-old plants for the propagation purposes All tools, such as blades, tweezers and petri dishes, were sterilized using an Autoclave at 121 ° C, 1.04 kg. The magnetic pieces were mounted on the outer surface corresponding to the shoots by the transparent adhesive tape and 1 cm away from shoots After 6 weeks; measurements were taken including the number and length of branches, the fresh and dry weight and the concentration of the main compounds of volatile oil. Least significant differences (LSD) were used to compare between means at 0.05level
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