Abstract
The effect of magnesium and polyamines (spermine, spermidine, putrescine and cadaverine) on the structure of yeast tRNA Phe has been investigated. It is found that magnesium induces structural changes and stabilizes hydrogen bonds in the temperature range 22–44°C in 0.17 M sodium. The number of Mg 2+ which affect tRNA structure increases from 1 ± 1 at 22°C to 4 ± 1 at 44°C and the number of additional base pairs formed in the presence of magnesium increases from 1 ± 1 at 22°C to 4 ± 1 at 44°C. The spectral changes are more-or-less sequential. The polyamine spermine stabilizes some, but not all, of the structural features stabilized by magnesium at 44°C, and the combination of magnesium and spermine, at low levels, is more effective than either cation alone in stabilizing tRNA structure. Comparison of the effects of spermine, spermidine, putrescine and cadaverine indicates that it is the asymmetric triamine unit which is important in the stabilization. Some spectral changes induced by magnesium can be assigned to stabilization of specific tertiary structure interactions and to alteration of stacking adjacent to U 8 · A 14.
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