Effect of macrophage-stimulating protein on the release of cytokine and oxidative stress of alveolar macrophages in cigarette exposure rats

Abstract

Objective To investigate the effect of mcrophage-stimulating protein (MSP)on the release of cytokine and oxidative stress of alveolar macrophages (AM) in cigarette exposure rats.Methods Cultured normal and cigarette exposure (1 month,2 months,3 months) rats AM were treated with different concentrations of MSP for 24 h.The levels of TNF-α,IL-8 and IL-1β in the supernatant of AM were determined with ELISA.The levels of malondialdehyde (MDA) and superoxide dismutase (SOD) in the supernatant of AM were assayed by chromatometry method.Results ①MSP increased TNF-α,IL-8and IL-1β levels in the supernatant of AM from normal and cigarette exposure rats in a concentrationdependent manner.After treated with MSP,the levels of TNF-α,IL-8 and I1-1β in the supernatant of AM from each cigarette exposure group were significantly higher than that in normal group ( P ＜0.05).With the cigarette exposure time extension,the levels of TNF-α,IL-8 and IL-1β in the supernatant of AM were increased in a time-dependent manner.②MSP increased the level of MDA and inhibited the level of SOD in the supernatant of AM from normal and cigarette exposure rats in a concentration-dependent manner.Compared with control group,the levels of MDA in the supernatant of AM from the cigarette exposure 2 months and 3 months groups rats were increased,but the levels of SOD were decreased.The level of MDA was increased and the level of SOD was decreased in a time-dependent manner with the cigarette exposure time extension.Conclusions MSP increases significantly the release of TNF-α,IL-8,IL-1β,MDA and inhibits the production of SOD in cultured normal and cigarette exposure rats AM in a concentrationdependent manner.These effects of MSP in cigarette exposure rats are more pronounced than in normal rats,and cigarette exposure the longer these effects are more obvious. Key words: Merophage-stimulating protein; Alveolar macrophage; Cytokine; Oxidative stress