The mechanism of bone marrow mesenchymal stem cell conditioned medium to reduce vascular endothelial cell apoptosis induced by cigarette smoke extract

Abstract

Objective To explore the impact of bone marrow mesenchymal stem cell (MSCs) conditioned medium (CM) on the oxidative stress and apoptosis of vascular endothelial cells induced by cigarette smoke extract (CSE).Methods Vascular endothelial cells and MSCs were cultured.Experiment was performed on three groups:control group,CSE group,and CM + CSE group.The apoptosis rate of vascular endothelial cells was assayed.The malondialdehyde (MDA),superoxide dismutase (SOD),the bax protein,B lymphocytes/leukemia-2 (bcl-2) protein and Caspase-3 in vascular endothelial cells were detected.Results The apoptosis rate in CM + CSE group was significantly reduced as compared with CSE group [(10.02 ±0.45) vs.(20.20 ± 1.29),P ＜0.01].The MDA activity in CM + CSE group was significantly reduced as compared with CSE group [(1.021 ±0.265) vs.(2.910 ±0.157),P ＜0.01].The SOD in CM + CSE group was significantly higher than in CSE group [(25.25 ±0.25) vs.(15.28 ± 1.37),P ＜0.01].The Caspase-3 expression in CM + CSE group was lower than in CSE group [(0.301 ± 0.165)vs.(0.791 ± 0.157),P ＜ 0.01].The positive expression rate of bax in CM + CSE group was lower than in CSE group [(16.32 ± 0.45) vs.(25.36 ± 1.22),P ＜ 0.01].The positive expression rate of bcl-2 in CM + CSE group was higher than in CES group [(12.25 ± 0.25) vs.(3.23 ± 0.57),P ＜ 0.01].Conclusion The CM from MSCs can partially inhibit the oxidative stress and apoptosis in endothelial cells by decreasing the levels of bax protein and Caspase-3 expression,and increasing the level of bcl-2 protein. Key words: Cigarette smoke extract; Vascular endothelial cell; Oxidative stress ; Apoptosis