Effect of LRIG3 gene overexpression on proliferation of glioma U251 and U87 cells and expression of PCNA and Ki-67

Abstract

Objective To explore the effect of overexpression of LRIG3 gene on proliferation of glioma cells and expression of proliferating cell nuclear antigen (PCNA) and Ki-67, and the possible mechanisms. Methods The plasmid containing LRIG3 gene and control was transduced into glioma U251 and U87 cells respectively. The mRNA and protein levels of LRIG3 were detected by using reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. Cell proliferation was examined by methyl thiazol tetrazolium (MTT) assay. The expression of PCNA and Ki-67 was detected by strept avidinbiotin complex (SABC). Results Compared with control cells, the mRNA levels of LRIG3 were raised by 67.6％ and 79.9％ in LRIG3 cells of U87 and U251, respectively, and the protein levels were increased by 62. 3％ (U87) and 91.0％ ( U251 ) respectively. Overexpression of LRIG3 resulted in the reduction of cell proliferation. The positive rate of PCNA was significantly lower in LRIG3 cells than in control cells [U87: (33.60±4.82)％ vs (55.50±4.01)％; U251: (27.49±3.17)％ vs (47.81 ±4.67)％ (P＜0. 05)]. The positive rate of Ki-67 was also significantly decreased in LRIG3 transduced cells as compared with control cells[U87 : (23. 50 ±4. 60)％ vs (55.20 ±4. 19)％ ; U251 : (24. 30 ± 3. 76) ％ vs (48. 50 ± 6. 11 ) ％ ( P ＜ 0. 01 )]. Conclusion Up-regulating LRIG3 gene expression can reduce the proliferation of glioma U87 and U251 cells. Key words: Glioma; LRIG3 ; Proliferating cell nuclear antigen; Ki-67