Abstract
Objective To explore the effects of INI1 on gastric cancer cell line SGC7901 proliferation and its molecular mechanisms.Methods The expression levels of INI1 in the samples of gastric carcinoma and surrounding tissue were detected by using quantitative reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting.The INI1-GFP plasmid and INI1-siRNA were transfected into gastric cancer cell line SGC7901 by LipofectamineTM 2000 transfection regent,then the expression levels of INI1 and proliferating cell nuclear antigen (PCNA) were detected by quantitative RT-PCR and Western blotting,and the SGC7901 cell proliferation activities were assayed by methyl thiazol tetrazolium (MTT) and cell number counting.Results The INI1 expression level in samples of gastric carcinoma was lower than that of surrounding tissue (P ＜0.01 ).The INI1-GFP transfection increased the INI1 expression dramatically,and the cell proliferation was suppressed significantly; At the same time,the expression of PCNA was down-regulated as compared with control group (all P ＜0.01 ).On the contrary,the INI1-siRNA transfection down-regulated the INI1 expression markedly,while the cell proliferation was increased significantly,and the expression of PCNA was up-regulated as compared with control group (all P ＜ 0.01 ).Conclusion INI1 is an inhibitor to gastric cancer cell proliferation probably by suppressing the PCNA expression. Key words: Gastric neoplasms; INI1 ; Proliferating cell nuclear antigen
Published Version
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