Effect of microRNA-1 on the expression of angiogenesis-related factors in gastric cancer cells

Abstract

Objective To observe the role of microRNA (miRNA, miR)-1 in the development of gastric cancer. Methods Real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) was used to detect the expression of miR-1 in human gastric cancer cell lines SGC7901 and BGC823. The plasmid expressing miR-1 was transfected into gastric cancer cells by transient transfection to detect the effect of miR-1 on the proliferation of gastric cancer cells. The effects of over-expressed miR-1 on angiogenesis-related factors vascular endothelial growth factor (VEGF)-A and EDN-1 in gastric cancer cells were detected by Western blotting. Double-reporter assay was used to analyze miR-1 interaction with VEGF-A and EDN1 3’untranslated regions (3’UTR). Results Compared with normal gastric mucosal epithelial cells, miR-1 was down-regulated in gastric cancer cells (P=0.000), and miR-1 inhibited VEGF-A and EDN-1 in proteins levels by binding to specific sites of VEGF-A and EDN-1 3’UTR, thereby inhibiting the proliferation of gastric cancer cells. Conclusion miR-1 inhibits tumors by inhibiting angiogenesis-related factors in human gastric cancer. Key words: Gastric cancer; MicroRNA-1; Vascular endothelial growth factor