Abstract
The longitudinal T 1 and transversal T 2 magnetic relaxation times of DNA solutions were measured at various ionic strength at elevated and lowered temperatures. From the dependence of T 1 and T 2 values on temperature the non-rotational hydration of DNA molecules in the native and denatured state and also during helix-coil transition was calculated. Dehydration of native DNA molecules was observed at elevated temperature. A higher ionic strength of DNA solutions is favourable for stronger dehydration at low temperatures. At temperatures close to the T m value, determined spectrophotometrically at 260 nm (73.5, 89.5 and 99° for DNA at 0.02, 0.15 and 1 M NaCl, respectively), the DNA molecules seem to be completely dehydrated. At lowered temperatures rehydration of denatured DNA molecules was observed. However, at each temperature the hydration of denatured DNA has an average value of only 42 % of that observed for native DNA.
Published Version
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