Effect of inoculum source (Sheep or Goat) on in vitro gas production and rumen fermentation parameters of some fodder shrubs in northern Tunisia

Abstract

In order to ensure nutritional characterization of some fodder shrubs and test the effect of inoculum source (sheep and goats) on their digestibility. Four shrubs “Acacia cyanophylla, Atriplex halimus, Opuntia ficus-indica. Var.inermis(cactus) and Medicago arborea” were tested in vitro by incubating them firstly into the rumen fluid of sheep then into goat’s inoculums. The evaluation included chemical composition, total gas production (CO2 and CH4), the prediction of organic matter digestibility (OMD), metabolizable energy (ME), the concentration of total volatile fatty acids (SCFA) and metering ammonia nitrogen (NH3-N). Ruminal fermentation of Opuntia ficus-indica and Medicago arborea have identified more total gas amonts than Atriplex halimus and Acacia cyanophylla. Digestibility of organic matter, metabolisable energy and the concentration of total volatil fatty acids were higher in the case of Opuntia ficus-indica compared to other shrubs. The lowest values were recorded in the Atriplex halimus. The concentration of ammonia nitrogen (NH3-N), it turned out that Opuntia ficus-indica is the food with less protein concentration (140 and 270.66 mmol/syringe) respectively for sheep and goats. In against part, the Medicago arborea is presented as a good protein source recording of ammonia nitrogen concentration (214.67 mmol/syringe) into the sheep’s rumen and (494.67 mmol/syringe) into the goats inoculum. The source of the inoculum showed a highly significant effect (p > 0.05).