Abstract

目的 观察白细胞介素-1α(IL-1α)对体外培养的牛小梁网细胞分泌基质金属蛋白酶(MMPs)和基质金属蛋白酶组织抑制剂(TIMPs)的影响,探讨IL-1α降低眼压的机制.方法 采用酶谱分析法和反向酶谱分析法检测牛小梁网细胞MMP和TIMPs的分泌,并观察IL-1α对MMP和TIMPs分泌的调节作用.结果 体外培养的牛小梁细胞分泌MMP-2、MMP-3、MMP-9和TIMP-1;IL-1α对MMP-2、MMP-3、MMP-9的分泌有促进作用,且呈时间和浓度依赖性,其中以IL-1α对MMP-3的分泌促进作用最强;IL-1α对TIMP-1的分泌也有微小的增加作用.结论 IL-1α能够显著促进牛小梁网细胞分泌MMPs,打破了MMP/TIMPs的平衡,可能进一步促进小梁网细胞外基质(ECM)的分解,增强房水流动性。

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