Abstract
To evaluate the effect of hyperbaric oxygenation (HBO) on the expression of the genes antioxidant glutathione peroxidase 4 (Gpx4) and lactoperoxidase (Lpo) in the lung of mice subjected to intestinal ischemia and reperfusion (IIR). Control group (CG) in which were subjected to anesthesia, laparotomy and observation for 120 minutes; an ischemia and reperfusion group (IRG) subjected to anesthesia, laparotomy, small bowel ischemia for 60 minutes and reperfusion for 60 minutes; and three groups treated with HBO during ischemia (HBOG + I), during reperfusion (HBOG + R) and during ischemia and reperfusion (HBOG + IR). Studied 84 genes of oxidative stress by the method (RT-qPCR). Genes with expression levels three times below or above the threshold cycle were considered significantly hypoexpressed or hyperexpressed, respectively (Student's t-test p<0.05). Gpx4 and Lpo were hiperexpressed on IRG, showing a correlation with these genes with lung oxidative stress. Treated with HBO, there was a significant reduction on genic expression on HBOG+I. Hyperbaric oxygenation showed to be associated with decreased expression of these antioxidant genes, suggesting a beneficial effect on the mechanism of pulmonary oxidative stress whenever applied during the ischemia.
Highlights
The phenomenon of intestinal ischemia and reperfusion (IIR) frequently occurs in connection with abdominal organ transplants, arterial and venous occlusive diseases of the digestive tract and circulatory shock[1]
Assuming that gene expression is quantitatively related to the need for proteins encoded by specific genes, the aim of this study was to investigate the expression of genes associated with oxidative stress and antioxidant defense in the lung tissue of isogenic mice after IIR and to concomitantly evaluate the effects of hyperbaric oxygenation (HBO) on the expression of these genes
In the intestinal tissue of the group that was subjected to intestinal ischemia and reperfusion (IRG), 73 (86.90%) of the 84 (100%) genes presented positive expression
Summary
The phenomenon of intestinal ischemia and reperfusion (IIR) frequently occurs in connection with abdominal organ transplants, arterial and venous occlusive diseases of the digestive tract and circulatory shock[1]. IIR is usually associated with the development of inflammation, microcirculatory dysfunction, and the production of reactive oxygen species (ROS) that damage local tissue as well as tissue in distant organs. The supply of an oxygen-rich environment (100% medical oxygen) under atmospheric pressure of two to three atmosphere absolute (ATA) favors the diffusion of O2 into plasma and bodily fluids. Under these conditions, hyperbaric oxygenation (HBO), by providing an increased concentration of O2 in bodily fluids, can decrease or even prevent the formation of ROS, even during circulatory compromise[4,5]. HBO may act by protecting tissues against the harmful effects of free radicals and disrupting the activation of pulmonary oxidative stress signaling pathways
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