Abstract
To investigate the effects of hyperbaric oxygenation (HBO) on intestinal ischemia and reperfusion (IR) injury, we evaluated the expression of 84 genes related to oxidative stress and the antioxidant response in mouse hearts. Four groups were subjected to 60 minutes of intestinal ischemia followed by 60 minutes of reperfusion: IRG, ischemia and reperfusion group without HBO; HBO-IG, which received HBO during ischemia; HBO-RG, which received HBO during reperfusion; and HBO-IRG, which received HBO during ischemia and reperfusion. The control group (CG) underwent anesthesia and laparotomy and was observed for 120 minutes. The (RT-qPCR) method was applied. Genes with expression levels three times below or above the threshold cycle were considered significantly hypoexpressed or hyperexpressed, respectively (Student's t-test p<0.05). Eight genes (9.52%) were hyperexpressed in the IRG. When the HBO groups were compared to the IRG, we found a decrease in the expression of eight genes in the HBO-IG, five genes in the HBO-RG, and seven genes in the HBO-IRG. The reduction in the expression of genes related to oxidative stress and antioxidant defense following HBO in mouse hearts resulting from intestinal IR injury was more favorable during the ischemic period than during the reperfusion period.
Highlights
Ischemia-reperfusion (IR) injuries with cellular alterations and humoral disorders lead to an imbalance in homeostasis[1]
Based on the assumption that gene expression is quantitatively related to the need for the protein that it encodes, the objective of the study was to investigate the expression of genes involved in oxidative stress and antioxidant defense in cardiac tissues in isogenic mice
In the cardiac tissues of the group subjected to intestinal ischemia and reperfusion (IRG), 28 (33.33%) genes had a positive expression, and 56 (66.66%) genes had a negative expression
Summary
Ischemia-reperfusion (IR) injuries with cellular alterations and humoral disorders lead to an imbalance in homeostasis[1]. These injuries occur as a result of infectious processes, arterial trauma, cardiac arrest, stroke, embolism, tumors, transplants, organ resections, myocardial infarction, and cardiovascular surgeries[2]. Because O2 deficiency triggers a chain of adverse events, the supply of O2 in a hyperbaric environment seems to be a novel and relevant option to interrupt the sequence of deleterious effects associated with IR5,6. Hyperbaric oxygenation (HBO) involves the supply of 100% oxygen (O2) in an environment with atmospheric pressure ranging from two to three absolute atmospheres (ATA). Plasma and body fluids will have an increased O2 concentration, regardless of the hemoglobin saturation, depending on the integrity of vascular circulation[7,8]
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