Effect of Humic Substances as Feed Additive on the Growth Performance, Antioxidant Status, and Health Condition of African Catfish (Clarias gariepinus, Burchell 1822).

Abstract

Simple SummaryGlobal aquaculture requires the development of new strategies to maintain the continuous growth of production, such as the development of aquafeeds with sustainable and functional components, ensuring better growth and health conditions of fish. Humic substances (HS) have the potential to become a functional additive for aquafeeds, as their growth-promoting and immunostimulant effects have been found in farm animals. Recently, there is limited knowledge on how different HS affects overall performance of various fish species. Therefore, in this study, the effects of four experimental Siberian leonardite HS diets (HS0, HS1, HS3, and HS6) on growth and production parameters, condition and somatic indices, overall mortality, health condition, and antioxidant status were assessed in juvenile Clarias gariepinus, which is well-known as a fast-growing and high-resistant fish species when reared at high stocking densities up to 500 kg m−3. In this study, growth and production parameters, condition, and somatic indices or mortality rate were not significantly affected by tested HS diets. On the other hand, moderately positive effects were observed regarding health status and good antioxidant parameters, especially in the HS3 group over the 56-day study.In the present study, a possible nature immunostimulant and growth promoter—humic substances (HS) originating from Siberian leonardite mineraloid—were tested on juvenile Clarias gariepinus performance. Feed additive was applied onto commercial pelleted feed at four HS levels—0, 1, 3, and 6% w/w (HS0, HS1, HS3, HS6, respectively). Diets were tested in five repetitions (in total, n = 1800 individuals, mean body weight 28.1 ± 6.2 g) for 56 days. Growth and production parameters, fish condition and somatic indices, and overall mortality were evaluated after 14, 28, 42, and 56 days of exposure. Whereas, plasma samples were collected only after 0, 28, and 56 days, when fish health status was assessed with biochemical parameters (total proteins, TP; alanine aminotransferase, ALT; aspartate aminotransferase, AST; lactate dehydrogenase, LDH; cholesterol, CHOL; triglycerides, TAG) and fish antioxidant status with glutathione (reduced glutathione, GSH; oxidized glutathione, GSSG; glutathione ratio GSH/GSSG). Although a significantly positive effect of HS feed additive on growth performance was not found in the present study, moderately positive effects were found regarding biochemical parameters (ALT, AST, LDH, CHOL, and TAG) and antioxidants (GSH/GSSG ratio) that were improved especially in the HS3 group.