Effect of Huangqi Guizhi Wuwu Decoction on rats with prolapse of lumbar intervertebral disc

Abstract

Purpose: This study aims to study the effect of Huangqi Guizhi Wuwu Decoction on the serum interleukin-β1 (TFG-β1) and tumor necrosis of experimental rats with lumbar disc herniation under the guidance of traditional Chinese medicine theory and modern scientific research methods. The influence of tumor necrosis factor -α (TNF-α) content. After establishing the rat lumbar disc herniation model, through 4 weeks of experimental intervention, the serum of various rats was collected, and the ELISA method was used to detect the content of TFG-β1 and TNF-α in the serum of each group of rats to obtain data Use statistical methods to analyze and process the data, explore the inflammatory factors that cause lumbar intervertebral disc herniation and the possible mechanism of action of Huangqi Guizhi Wuwu Decoction on the treatment of lumbar intervertebral disc herniation, and provide the prescription for the treatment of lumbar intervertebral disc herniation in the future Scientific experimental basis. Methods: The 48 SD male rats used in the experiment, weighing between 160-200g, were randomly divided into 4 groups, namely, A is the blank control group, B is the model, C is the Huangqi Guizhi Wuwu Decoction experimental group, and D It is the western medicine group of ibuprofen suspension, the blank group does not do any treatment, the other 3 groups of rats under aseptic conditions cut the skin (about 4cm in length) and subcutaneous tissue in the middle of the back, and bluntly separated the paravertebral tissue from the left spine Muscles, with bilateral iliac spine as anchor points, remove the spinous processes, left lamina and articular processes of the two vertebrae above the iliac spine. Expose the dura mater on the left side under an operating microscope to confirm the left spinal nerve. Take the jelly-like intervertebral disc nucleus pulposus from the excised tail of the mouse and place it gently around the dorsal side of the left nerve root. 3d intraperitoneal injection of penicillin sodium 2×105U/kg, once a day. Two weeks after modeling, imaging and histopathology showed obvious inflammatory cell infiltration. The infiltrating inflammatory cells were mainly neutrophils and macrophages. The nerve tissue was swollen and the nerve myelin sheath was swollen, ruptured, and axons. Swelling or disappearance, Schwann cells autolyzed and softened, and successfully established a rat lumbar disc herniation model. The administration was started on the first day after successful modeling. The frequency of administration in each group was once a day. The dosage was as follows: the blank group and the model group were given normal saline (1ml|100g) respectively, and the experimental group was given Huangqi Guizhi Wuwu Decoction was given by intragastric administration (1ml|100g), the western medicine group was given ibuprofen suspension (1ml|100g) by intragastric administration, and 1/4 animals were randomly sacrificed on the 7, 14, 21 and 28 days after intragastric administration. Three rats per group each time, blood was taken from the heart, centrifuged at 3000 r/min for 15 minutes, and the supernatant was drawn. The serum TFG-β1 and TNF-α contents of each group of rats were detected by ELISA. Results: 1. Changes in the level of TFG-β1 in serum: 7th, 14th, 21st, and 28th days after intragastric administration, the detection of serum TFG-β1 levels in each group showed that there was no significant change in TFG-β1 levels in group A; group B Compared with group A, the level of TFG-β1 increased abnormally; group C and group D decreased the level of TFG-β1, but group C dropped more significantly than group D, and the difference between the two groups was statistically significant (P<0.05). 2. Changes in serum TNF-α levels: There was no significant change in serum TNF-α levels in group A rats; serum TNF-α levels in group B rats were significantly increased, and serum TNF-α levels in group C and D rats Compared with group B, the levels of TNF-α in group C decreased more significantly than those in group D. The difference in serum TNF-α levels between the two groups of rats was statistically significant (P <0.05). Conclusions: 1. An animal model of rat lumbar disc herniation can be established by rat autologous nucleus pulposus transplantation;2. Before treatment, the serum TFG-β1/TNF-α content in rats in group C and D increased significantly, suggesting that the content of serum TFG-β1/TNF-α may be related to the incidence of lumbar disc herniation;3. Huangqi Guizhi Wuwu Decoction can significantly reduce the content of TFG-β1 and TNF-α in the serum of rats with lumbar disc herniation, and the effect is better than that of the western medicine group.Tabled 1Serum TFG-β1 levels (pg/ml) of rats in each group on 7, 14, 21, 28 days after gavageGroup7d14d21d28dB Group58.31±23.7959.60±24.5861.12±12.9363.17±13.38C Group43.11±22.8438.27±34.3226.35±11.9815.86±24.47*D Group57.26±13.1149.33±23.7435.46±23.0926.12±22.83* Open table in a new tab Tabled 1Serum levels of TNF-α (ng.L-1) in rats of each group at 7, 14, 21, 28 days after gavageGroup7d14d21d28dB Group89.67±28.8288.64±32.0890.32±22.3391.17±13.78C Group69.44±40.7957.73±32.8850.60±15.4339.25±21.58*D Group76.86±33.0165.23±33.4658.26±22.8947.52±26.13* Open table in a new tab