Effect Of Glucose Intake On Blood Glucose And Tissue Glycogen Levels In Hypoxia Exposed Rats

Abstract

Listen

Translate article

PURPOSE: This study was aimed to examine the effect of glucose intake on blood glucose and heart, liver and skeletal muscle glycogen levels with 14% normobaric systemic hypoxia exposure in rats. METHODS: Eighty SD rats were divided into 10 groups such as, fasting hypoxia 0 min (FH-0, n=8), fasting hypoxia 30 min (FH-30, n=8), fasting hypoxia 60 min (FH-60, n=8), fasting hypoxia 120 min (FH-120, n=8), fasting hypoxia 240 min (FH-240, n=8), oral glucose hypoxia 0 min (GH-0, n=8), oral glucose hypoxia 30 min (GH-30, n=8), oral glucose hypoxia 60 min (GH-60, n=8), oral glucose hypoxia 120 min (GH-120, n=8), and oral glucose hypoxia 240 min (GH-240, n=8). RESULTS: The blood glucose concentration was significantly increased in fasting hypoxia 30 min compared to FH-0 min (FH-0 = 67.67±5.28 mg/dl<FH-30 = 83.86±2.93 mg/dl). No effect on red, white gastrocnemius muscles and liver glycogen levels in the fasting hypoxia group at all time points. However, heart glycogen levels were significantly (p<0.05) increased in fasting hypoxia group after 30min (FH-30=41.86±3.07 μmol/g) and 240min (FH-240=40.48±1.40 μmol/g) when compared to zero min (FH-0 32.26±2.84 μmol/g). After oral glucose administration red gastrocnemius and heart glycogen levels were significantly increased at 240min (GH-0=31.03±4.74 μmol/g < GH-240=44.87±1.10 μmol/g; GH-0=34.36±2.44 μmol/g < GH-240=58.63±6.16 μmol/g, respectively), but no effect in white gastrocnemius muscle glycogen levels. Liver glycogen levels were increased at 60, 120 and 240min (GF-60=75.92±8.51 μmol/g, GF-120=88.32±12.99 μmol/g, GF-240=96.99±12.81 μmol/g) after oral glucose intake compared that of zero min. CONCLUSIONS: Hypoxia under fasting condition increase heart glycogen levels. Hypoxia along with oral glucose intake induces liver glycogen response faster than heart and oxidative red gastrocnemius muscle, but no effect on white gastrocnemius muscle.