Effect of Gallic Acid Treatment on Hepatic Tissue in Ischemia-Reperfusion

Abstract

Aim: Hepatic IR occurs in many clinical conditions such as liver injury, liver hemorrhage and shock, surgical resection, and transplantation. In our study, it is aimed to investigate whether gallic acid (GA) can be used for hepatoprotective purposes, histopathologically.
 Study Desıgn: 24 Wistar albino male rats were divided into 3 groups (n:8). Surgical procedures were done under general anesthesia. A 4 cm midline abdominal incision were opened to observe hepatoduodenal ligament. The portal vein and hepatic artery was closed with a rubber band and silk suture, and ischemia was performed for 60 minutes. The suture was opened and hepatic reperfusion was performed for 6 hours. 50 mg/kg Gallic Acid was administered to the rats in this group by intraperitoneal route once a day for 14 days.
 Results: Normal histology of lvier was observed with regular vena centralis, endothelial cells, hepatocytes and sinusoid. In IR group, blood vessels were dilated and congested with cell infiltrations. Hepatocytes were degenerated with irregular sinusoides and hyperplasic Kupfer cells. IR+GA group, mononuclear cell infiltration continued with thickening of the basement membrane of the vessels. Vacuolated hepatocyte was observed. GA treatment alleviasted the pathologies occurred due to IR injury.
 Conclusion: It was thought that the cellular damage in the inflammation signal, which started as a result of the cessation of blood flow after ischemia-reperfusion injury, affects the classical lobule of the liver especially around the vena, and may cause necrotic changes by increasing cell apoptosis due to functional nutritional deficiency.
 Keywords: liver, ischemia, reperfusion, gallic acid, histology