Effect of fluorophore linkage position of [formula omitted] fatty acids on probe distribution between coexisting gel and fluid phospholipid phases

Abstract

The quenching of probe fluorescence by spin-labeled phospholipid has been used to determine the distribution of a series of n-(9- anthroyloxy) fatty acids between coexisting gel and fluid liquid-crystal phases in multilamellar phospholipid vesicles. The phase distribution ratio in every case is found to favor the fluid lipid phase, but is much greater between fluid and Ca 2+-induced gel than between fluid and thermal gel. For a given gel type, n-(9- anthroyloxy)stearic acids with n=3, 6, 9 or 12 as well as 11-(9-anthroyloxy)undecanoic acid all exhibit similar behavior, favoring the fluid phase by about a factor of 4 over thermally-induced lipid gel phase and by 18 over Ca 2+-induced gel phase. 16-(9-Anthroyloxy)palmitic acid, with the bulky probe at the terminus of the 16-carbon chain, favors the fluid phase less strongly, by a factor of 1.5 or 11 over thermally-induced or Ca 2+-induced gel phase, respectively, indicating better packing of this probe in phospholipid gel phases.