Effect of Fluopyram on Pratylenchus penetrans on Corn in the Field and In Vitro.

Abstract

The effects of a fluopyram seed treatment on lesion nematodes (Pratylenchus spp.) and other plant-parasitic nematodes (PPNs) were evaluated on corn in multiple field locations in 2020 and 2021. The highest rate of fluopyram seed treatment (0.15 mg seed-1) reduced early season population density of lesion nematodes compared with the base treatment control in 2020 only. However, fluopyram did not affect late season lesion nematode population density and corn yields. Fluopyram seed treatment also had minimal or nonsignificant effects on other PPN species. Based on these results, the effects of fluopyram were tested in vitro on Pratylenchus penetrans. Results demonstrated that fluopyram severely affected motility in P. penetrans. The sensitivity of P. penetrans second-stage juveniles (J2s) to fluopyram was significantly higher than at J4 and adult, suggesting that sensitivity to fluopyram is dependent on developmental stage. In addition, the effects of fluopyram were reversible at an EC50 but were irreversible at the maximum concentration (25 μg/ml). Overall, our results indicate that fluopyram has potential for controlling P. penetrans, but its efficacy is variable depending on nematode developmental stage and chemical concentration. Further research is needed to determine if these impacts can translate to field scenarios.