Abstract
Objective To investigate the effect of endogenous electric fields on proliferation and migration of epidermal stem cells (EpSCs) in vitro. Methods EpSCs in the first passage were cultured on simulated endogenous electric field. Galvanic stimulation set at an intensity of 0 mV/mm (GroupⅠ), 100 mV/mm (GroupⅡ) and 200 mV/mm(Group Ⅲ) was given twice a day for 3 days with each time continuing1 hour and resting 1 hour. Effect of bio-electric field on cell proliferation was measured by direct cell counting in fixed visual field. Repeated experiment but continued stimulation for 3 hours was performed. Live cell migration was monitored to determine the effect of bio-electric field on the process of cell migration. Results For the EpSCs cultured in endogenous bio-electric simulation platform for 24, 48 and 72 hours, cell proliferation rate was the highest in Group Ⅲ[(107.4±21.9)%, (270.2±71.4)%, (544.0±95.1)% ] (P<0. 01). And higher cell proliferation was observed in Group Ⅰ[(35.1±21.0)%, (138.6±31.0)%, (323.5±23.0)%] than in Group Ⅱ[(66.9±24.2)% , (192.1±36.2)%, (406.7±50.7)%] (P<0. 01). After continued galvanic stimulation for 3 hours, cells in Groups Ⅱ and Ⅲ oriented towards cathode, but cells in Group Ⅰ moved randomly. In measurements of track velocity (Vt), displacement velocity (Vd) and electric field migration rate (Vx), Group Ⅲ revealed increased values[(42.5±2.8), (32.3 ±1.8), (29.7±1.3)μm/h] as compared with GroupⅠ[(36.2±2.2), (23.6±2.9), (18.2±1.8)μm/h] and Group Ⅱ[(34.3±1.6), (23.8±1.2), (21.2±1.6)μm/h] (P<0.01). Conclusion In vitro experiments reveal that endogenous electric fields can promote the proliferation of EpSCs and induce oriented movements towards the cathode. Key words: Stem cells; Cell proliferation; Cell movement; In vitro
Published Version
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