Research of Luteoloside in promoting proliferation and migration of human epidermal stem cells

Abstract

Objective To investigate the effects of Luteoloside on the proliferation and migration of human epidermal stem cells (EpSCs) and the proliferation of epidermal cells in skin tissue in vitro. Methods EpSCs were isolated from human skin tissue by type Ⅳ collagenase digestion and collagen fast adherence. EpSCs were identified by immunofluorescence staining of CK19 and β1 integrin. The effects of Luteoloside (0 to 5.0 μmol/L) on the proliferation of EpSCs were measured by MTT assay. The effects of Luteoloside on EpSCs migration and wound healing were observed by scratch-wound model in vitro. The effects of Luteoloside on the thickness of epidermis in vitro were observed by HE staining. Results EpSCs formed a cobblestone-shaped cell monolayer when cultured on a collagen coated dish. Immunofluorescence staining showed that more than 90% cells were CK19 and β1 integrin positive. Luteoloside at 0.1 to 1.0 μmol/L could significantly promote the EpSCs proliferation; Luteoloside at 1.0 μmol/L could significantly enhance EpSCs migration and scratch healing. Culture of skin tissues with 1.0 μmol/L Luteoloside for 5 days could significantly increase the thickness of the epidermis. Conclusion Luteoloside can promote the proliferation and migration of EpSCs, promote the proliferation of epidermal tissue, and play an important role in skin self-renewal and injury repair. Key words: Cell proliferation; In vitro; Luteoloside; Epidermal stem cells; Migration