Effect of Dissolved Oxygen on Monoclonal Antibody Production from Hybridoma Cultured in Haemodialysers

Abstract

A bench scale cell culture system was constructed from commercially available materials that permitted the growth of a murine hybridoma cell line, 7F1C8. Following optimisation of nutrient requirements and serum supplementation, 7F1C8 cells were cultured in the extracapillary space (ECS) of a commercial haemodialyser for a period of 14 days at a dissolved oxygen tension (DOT) of 60% air saturation. At 14 days, the DOT was either decreased to 30% or increased to 75% air saturation and the culture continued for a further 24 days. Monoclonal antibody (MAb) production from the hybridoma culture maintained at a DOT of 30% air saturation over this period, produced 57.4% more MAb than the hybridoma culture maintained at a DOT of 75% air saturation. Examination of nutrient consumption and metabolite production indicated that the energy required for increased MAb production under low DOT conditions was derived from the anaerobic metabolism of glucose and not from the aerobic respiration of glutamine.