Abstract
A CHO cell line engineered to express and secrete the 75 kDa rat peptidylglycine α-amidating enzyme was adapted to serum-free medium and studied in three distinct culture systems: adherent cells in hollow fiber or microsphere fluidized bed perfusion bioreactors and suspension-adapted cells in a stirred tank bioreactor. Key parameters measured during growth and production phases included glucose and lactate concentrations, dissolved O2 and α-AE concentration. Performance parameters during 20 days of production phase with 1−2×1010 cells in each bioreactor were compared. Stirred tank fermentation of the rCHO cells provides the most efficient approach to production of reagent α-AE for use in the manufacture of amidated peptides.
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