Effect of α,α'-dipyridyl on Exogut Formation in Vegetalized Embryos of the Sea Urchin: (sea urchin embryo/exogut formation/vegetalized embryo/peptidyl praline hydroxylation/α, α'-dipyridyl).

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In presumptive vegetalized embryos, obtained by 3-hr treatment with chloramphenicol at the 16-32 cell stage, the rates of [14 C]proline incorporation into the collagen fraction and production of the [14 C]hydroxyproline residues increased during development between 16 hr (equivalent to mesenchyme blastula stage) and 40 hr (the early pluteus stage) after fertilization at 20°C. In presumptive vegetalized embryos, the radioactivity of [14 C]hydroxyproline residues was higher at the mesenchyme blastula stage (16 hr after fertilization), but lower at the post-gastrula stage than in normal embryos. In normal embryos at the post-gastrula stage, [14 C]hydroxyproline residues were mainly found in isolated spicules, and the amounts of [14 C]hydroxyproline residues in other parts were much lower than in vegetalized embryos, which had few, if any, spicules. α, α'-Dipyridyl, an inhibitor of prolyl hydroxylase, inhibited the hydroxylation of [14 C]proline residues in presumptive vegetalized and normal embryos, and blocked the formation of the archenteron and exogut.