Effect of Demineralized Dentin Matrix Graft alone and Combined with Statin or Propolis on Bone Repair in Rabbits’ Tibia

Abstract

Background: Demineralized dentin matrix (DDM) is a biologically active tissue could stimulate new bone tissue formation then resorbed during the bone remodeling process. Aim: The aim of the present study was to evaluate the effect of demineralized human dentin matrix graft alone and when combined with Statin or Propolis on bone repair in rabbits’ tibia. Materials and Methods: Thirty adult male rabbits (weight 2 to 2.5 kg) were used in this study. In each rabbit four holes were created (two in each tibia), one was left empty as control, the second was packed with DDM slices, the third was packed with DDM slices saturated with statin and the fourth was packed with DDM mixed with propolis. The animals were sacrificed at 2, 4 and 6 weeks postoperatively and the bone specimens were processed for histomorphometric analysis and scanning electron microscopic examination. Results: Histomorphometric analysis showed significantly high mean bone area for DDM/Statin group followed by DDM group then DDM/Propolis group then control group. SEM findings were supportive to these results. Conclusions: It was concluded that the DDM was profound and biocompatible material for bone repair. Addition of Statin promotes bone repair process while Propolis retards bone repair especially in early stages.