The Effect of Human Demineralized Dentin Matrix Scaffold Alone and Combined with Concentrated Growth Factor on Bone Repair in Rabbits

Abstract

Purpose: This study aimed to evaluate the potential effect of Human Demineralized Dentin Scaffold (HDDS) alone and when combined with Concentrated Growth Factor (CGF) on repair of bony defects in the tibia of rabbits, histologically, by using scanning electron microscope and computed tomography. Materials and Methods: Ten adult male rabbits (weight 2 to 2.5 kg) were used in this study. Ethics committee approval of Faculty of Dental Medicine For Girls as obtained with code number (REC-BI-19-01). In each rabbit two holes were created (one in each tibia). In five rabbits, the first control hole was left empty and the second was packed with CGF. In the other five rabbits, one hole was packed with DDM slices from anonymous human permanent teeth without carious lesion or other pathology and the second was packed with DDM and CGF. The rabbits were euthanized at 1 and 6 weeks postoperatively and the bone samples were processed for histological analysis. Results: Faster bone repair was occurred in the experimental groups. In all groups, Osteoid tissue formation was occurred at one week, and osseous tissue formation was seen filling the bone defects at 6 weeks. The best results after 6 weeks appeared in surgical bone defects grafted with HDDS and CGF where the new bone showed better organization than the HDDS alone and CGF alone and most of the formed osteons were fully compacted. Conclusion: Combining Human Demineralized Dentin Scaffold (HDDS) with concentrated growth factor (CGF) improved graft biomaterial when used as xenograft and it became more effective through inducing a faster bone repair.