Effect of Copper and Zinc on the Bioactivity and Cells Viability of Bioactive Glasses

Wers E,Bunetel L

doi:10.4172/2090-5025.s1-013

Abstract

Bioactive glasses, doped with traces of copper (Cu) and zinc (Zn) were synthesized by fusion method. Cu and Zn present interesting functions for the biological metabolism through their antibacterial, anti-inflammatory and antifungal properties. Several physical methods were employed to characterize the bioactive glasses before and after immersion in a Simulated Body Fluid (SBF). The “in vitro” experiments showed that after soaking in SBF, the behavior of bioactive doped glasses are different compared to pure glass 46S6. Obtained results show that glass matrix undergoes some changes after 15 days of immersion. The non toxic character of doped glasses was confirmed after 24 hours of incubation. The kinetic of release of Cu and Zn was carried out. It highlights that Cu is more released than Zn. By SEM, the morphology of hydroxyapatite obtained with Zn-doped glass show a better crystallization compared to Cu-doped glass.

Highlights

Synthetic biomaterials are considered like a great option to replace bone grafts
Bioactive glasses are able to form a hydroxyapatite layer Ca10(PO4)6(OH)2 on their surface when they are immersed in a simulated body fluid
The amorphous structure of all glasses was confirmed by XRD analyses before immersion in the Simulated Body Fluid (SBF)

Summary

Introduction

Synthetic biomaterials are considered like a great option to replace bone grafts. Bioactive glasses are able to form a hydroxyapatite layer Ca10(PO4)6(OH) on their surface when they are immersed in a simulated body fluid. This formation induces a bone bonding improving the bone growth [1,2]. Copper and zinc are interesting elements for biomedical applications because they present physiological properties. Copper has anti-inflammatory, anti-infectious and anti-bacterial properties. It is necessary for a lot of biological processes as angiogenic response [3]. Zinc introduces in several enzymatic processes, inhibits the bone resorption and promotes the proliferation of osteoblast [4]. The aim of this work is to study the effect of Cu and Zn on the formation of hydroxyapatite

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Conclusion