Abstract
Objective : To study the effect of composite resin monomer BisGMA on human dental pulp stem cell (hDPSCs) odontoblasts differentiation, and explore the effect of composite resin filling teeth on dentin formation. Material and Methods : separate and culture hDPSCs, identify cell phenotype by flow cytometry, and induce staining to assess multidirectional differentiation potential. The hDPSCs was stimulated with different concentrations of monomeric BisGMA, and the odontoblast differentiation capacity of each group was determined by CCK-8 assay, alkaline phosphatase activity detection, and q-PCR. Results: CCK-8 showed that the addition of 0.5 mM —— 8 mM BisGMA affected the cell increment, but no significant difference; the concentration of 0.5 mM —— 8 mM BisGMA reduced the cellular alkaline phosphatase activity and further decreased the activity with increasing concentration.The q-PCR results also showed that monomeric BisGMA inhibited the dentin-related gene OC (osteocalcin), DSPP (dentin sialophosphoprotein)Expression, and with the concentration increases, the inhibition is more obvious of. Conclusion : Composite resin monomer BisGMA has a negative effect on odontogenic differentiation of human dental pulp stem cells
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